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Microbial Cell Factories | Home page – Efficient recombinant expression and secretion of a thermostable GH26 mannan endo-1,4-β-mannosidase from
11/4/2010 · A zymogram of mannan endo-1,4-β-mannosidase activities was generated by an in-gel activity assay using 0.25% locust bean gum as substrate, copolymerized with 10% (w/v) polyacrylamide. The enzyme samples were mixed with the loading buffer in the
Quality control of inclusion bodies in Escherichia coli | Microbial Cell Factories | Full Text
28/5/2010 · Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) For analysis of the IBs the cells were disrupted by French Press with 900 PSIG (62.1 bar) followed by centrifugation for 10 min at 14,000 rpm.
Extraction, purification and analysis of histones | Nature Protocols
7/6/2007 · 2D TAU gels can be run with a homemade SDS-PAGE gel as second dimension or with a pre-cast commercial gel with a '2D' well, such as the NuPAGE 10–20% SDS gel (Invitrogen, cat. no. EC6136).
Biodegradation of flonicamid by Ensifer adhaerens CGMCC 6315 and enzymatic characterization of the nitrile hydratases involved | Microbial Cell
13/7/2021 · The concentrations of the separating gel and focusing gel were 12.5% and 5% (w/v), respectively. The optimal reaction pH and temperature for degradation of FLO were determined by measuring NHase activity in different buffers at pH 4–9 (citrate
Production of biologically active human interleukin-10 by Bifidobacterium bifidum BGN4 | Microbial Cell Factories | Full Text
19/1/2021 · Background Bifidobacterium spp. are representative probiotics that play an important role in the health of their hosts. Among various Bifidobacterium spp., B. bifidum BGN4 exhibits relatively high cell adhesion to colonic cells and has been
The N-terminus of Lactobacillus amylovorus feruloyl esterase plays an important role in its secretion by Lactobacillus plantarum and Escherichia
3/8/2021 · Background Feruloyl esterase is a multifunctional esterase with potential industrial applications. In the present study, we found the Lactobacillus amylovorus feruloyl esterase (FaeLam) could be secreted by L. plantarum and Escherichia coli.
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Dynamic control of the distribution of carbon flux between cell growth and butyrate biosynthesis in Escherichia coli – Springer
11/6/2021 · Abstract Microbial cell factories offer an economic and environmentally friendly method for the biosynthesis of acetyl-CoA-derived chemicals. However, the static control of carbon flux can cause direct and indirect competition for acetyl-CoA
Protein Profiles of Aspergillus Species Isolated From the Tea Gardens and Factories Air in Northern Iran | Jundishapur Journal of Microbiology
1/11/2012 · The extracts obtained were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) to separate the fungal antigenic proteins. Results: A total of 4 157 fungal colonies were observed in the tea gardens air and 3 950
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