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10% Mini-PROTEAN ® TBE-Urea Gel, 15 well, 15 µl ® 4566036 Pkg of 2, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN electrophoresis cells

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8. Gel purification of cDNA 8. Gel purification of cDNA 8.1. Spin down and wash the samples (see 7.1), then resuspend the pellets in water (6 μL). 8.2. Add 2x TBE-urea loading buffer (6 μL) and heat samples to 80 C for 3 min directly before

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A Guide to Polyacrylamide Gel Electrophoresis and Detection | Sai Ananth – Academia.edu

For protein separation, virtually In gel electrophoresis, proteins do not all enter the all methods use polyacrylamide as an anticonvective, gel matrix at the same time. Samples are loaded sieving matrix covering a protein size range of into

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Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loading | Nature Protocols

31/7/2014 · Denaturing polyacrylamide gel solution, 500 ml, 15% (wt/vol) Prepare stock solution by dissolving 210 g of urea in 50 ml of 10× TBE and 187.5 ml of 40% (19:1) acrylamide.

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22/6/2012 · Ready-to-Use Gel System for 6% Denaturing Gels UreaGel-6 EC-836 Convenient system for casting 19:1 6% denaturing gels with Acrylamide, Bis, TEMED, TBE and urea. (pg. 10) TBE 10X EC-860 Formulated

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