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Chapter 16 Resolution of Histonies by Polyacrylamide Gel Electrophoresis in Presence of Nonionic Detergents – ScienceDirect

1/1/1978 · ELECTROPHORESIS OF HISTONES IN TRITON X-IOO 231 IV. Applications Polyacrylamide gel electrophoresis in the presence of nonionic detergents is capable of resolving variants or mutants differing only in the substitution of a single neutral amino

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Disaggregation of adenylate cyclase during polyacrylamide-gel electrophoresis in mixtures of ionic and non-ionic detergents

1. Adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] solubilized from the rat liver plasma membrane with 1% Lubrol PX and partially purified by gel filtration in buffer containing 0.01% Lubrol PX was physically characterized by

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Effects of triton X-100 on gel electrophoresis and gel chromatography of histones. Possible binding to helical regions – PubMed

Polyacrylamide gel electrophoresis of whole histones of calf thymus, chicken erythrocytes, and Tetrahymena was carried out in the absence or presence of a nonionic surfactant, Triton X-100 (up to 6 mM), in 0.9 M acetic acid and 6.25 M urea-0.9 M

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Polyacrylamide gel electrophoresis – Alfa

Polyacrylamide gel electrophoresis Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a free-radical

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Disaggregation Adenylate Cyclase Polyacrylamide-Gel Electrophoresis Mixtures Ionic Non-Ionic Detergents

usefulness of polyacrylamide-gel electrophoresis to detect and overcome aggregation problems with membraneproteins and suggest that detergent mixtures in specific ratios may be useful in the purification of adenylate cyclase and other intrinsic

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Coating of pre-cast electrophoresis slab gels – Bio-Rad Laboratories, Inc.

1. A pre-cast polyacrylamide slab gel for use in slab gel electrophopresis, said pre-cast gel comprising: a pair of chemically inert, transparent plates of a member selected from the group consisting of glass and polystyrene-acrylonitrile blend

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Pre-cast electrophoresis slab gels with extended storage life – Bio-Rad Laboratories, Inc.

12. A pre-cast polyacrylamide slab gel in accordance with claim 11 in which said nonionic amphiphilic polymer has a molecular weight of from about 100,000 to about 8,000,000. 13. A pre-cast polyacrylamide slab gel in accordance with claim 11 in

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Electrophoresis in gels containing zwitterionic groups – Hjelmeland – 1981 – ELECTROPHORESIS – Wiley Online Library

, Chapter 13 Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), The Proteome Revisited Theory and Practice of all Relevant Electrophoretic Steps, 10.1016/S0301-4770(01)80045-2, (217-274), (2001).

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Exercise 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) | Request PDF

Polyacrylamide gel electrophoresis (PAGE) can thus be consider as a major protein purification technique, allowing the separation of proteins according to their mol wt, charge, and geometric

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Purification of membrane proteins from Acholeplasma laidlawii by agarose suspension electrophoresis in Tween 20 and polyacrylamide and dextran gel

10/4/1975 · 1. J Biol Chem. 1975 Apr 10;250(7):2463-9. Purification of membrane proteins from Acholeplasma laidlawii by agarose suspension electrophoresis in Tween 20 and polyacrylamide and dextran gel electrophoresis in detergent-free media. Johansson KE,

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Two-dimensional gel electrophoresis

Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. 2-DE was

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Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones | SpringerLink

Abstract Acid-urea polyacrylamide gels are capable of separating basic histone proteins provided they differ sufficiently in size and/or effective charge (see Chapter 16).Schwager, S. L. U., Brandt, W. F., and Von Holt, C. (1983) The isolation

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