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Gelatin zymography protocol | Abcam

Gelatin zymography. Running the gel. Dilute conditioned media so that all samples have the same protein concentration. F or each sample, test one aliquot at a low protein concentration (5 µg/mL) and one at a high protein concentration (15

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Polyacrylamide Gel Electrophoresis | Cleaver Scientific

Polyacrylamide gel electrophoresis (PAGE) is a technique use almost universally in life science laboratories. The goal of this technique is to separate a mixed sample of proteins to identify and quantify single proteins from the mixture. The

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Laemmli SDS sample buffer, reducing (6X) – J61337 – Alfa Aesar

Further, it is used for polyacrylamide protein gel analysis. Notes Contains 375mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 9% beta-mercaptoethanol, 0.03% bromophenol blue.

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Bountigel-G | CarbonNeutralAgSciences

BountiGel G. BountiGel G is the granular version of the BountiGel product line. It was developed using a proprietary, patented technology that results in an eco-friendly, super-absorbent soil amendment that absorbs up to 150 times its weight in

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Western Blotting Protocol for Characterizing Extracellular Vesicles | STEMCELL Technologies

Load the desired volume (e.g. 35 µL) of samples per well in a 10% polyacrylamide gel. Run SDS-PAGE for 30 minutes at 200 V. Rinse gel twice with distilled water. Incubate gel in cold Transfer Buffer for 15 minutes. IV. Immunoblotting and Imaging

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Sodium Dodecyl Sulfate, Molecular Biology Grade (SDS)

Sodium Dodecyl Sulfate, Molecular Biology Grade (SDS), is a detergent that is known to denature proteins. It is used in denaturing polyacrylamide gel electrophoresis for the determination of protein molecular weight. It is also used in nucleic

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Protocols – Western Transfer (Western Blot)

Protocols – Western Transfer (Western Blot) Western Transfer, also known as Western Blotting, is a rapid immunoblotting technique for identifying the presence of a particular protein in a complex mixture of proteins such as cell lysates or sera.

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TBE Buffer for Agarose Gel Electrophoresis

TBE Buffer (5X) is a solution used in Agarose Gel Electrophoresis (AGE) typically for the separation of nucleic acids (i.e. DNA and RNA). Tris-Borate-EDTA (TBE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but

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Gel Loading Pipette Tip | STEMCELL Technologies

Axygen® Gel Loading Pipette Tips are ideal for accurate gel loading with precise control. They are suitable for use in gel electrophoresis involving agarose or polyacrylamide gels. These non-sterile polypropylene pipette tips fit most

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Cresol red, Triarylmethane dye (CAS 1733-12-6) (ab146319) | Abcam

Triarylmethane dye commonly used with lead acetate for the detection of urease after polyacrylamide gel electrophoresis (PAGE). Also used as a pH indicator and as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) electrophoresis.

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Laemmli SDS sample buffer, non-reducing (6X) – J60660 – Alfa Aesar

Laemmli SDS sample buffer, non-reducing (6X) is an electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Composition of this buffer is similar to the reducing buffer minus mercaptoethanol. This product is

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Gel Drying Kit – Promega

The Gel Drying Film is essentially gas-impermeable when dry. Dried gels may be scanned densitometrically and also projected using an overhead projector. A set of Gel Drying Frames will accommodate one standard 16 × 16cm polyacrylamide gel, four

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Laemmli SDS sample buffer, reducing (4X) – J60015 – Alfa Aesar

Further, it is employed in the polyacrylamide gel analysis of proteins. In addition to this, it is utilized to reduce disulfide bonds in order to denature proteins. Notes Contains 250mM Tris-HCl(pH 6.8), 8% SDS, 40% glycerol, 8% beta-mercaptoeth

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Blue/Orange Loading Dye, 6X

Blue/Orange Loading Dye, 6X, is a convenient marker dye containing 0.4% orange G, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 15% Ficoll® 400, 10mM Tris-HCl (pH 7.5) and 50mM EDTA (pH 8.0). It is provided in a premixed, ready-to-use form.

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Blue native electrophoresis protocol | Abcam

Add 7.5 µL 10% LM and incubate on ice for 30 min. Centrifuge 72,000 x g at 4°C for 10 min. Add 2.5 µL of a 5% suspension of Coomassie blue G in buffer A. Load samples on 6 – 13% native acrylamide gradient gel. Gel recipe and electrophoresis

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DL-Dithiothreitol, 99% (CAS 3483-12-3) | Glentham Life Sciences

Buy GC1199 – DL-Dithiothreitol, 99% (3483-12-3) online from Glentham Life Sciences, a manufacturer and supplier of fine chemicals. View catalogue prices, chemical data, technical specifications and MSDS documents.

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Acrylamide, 98% (CAS 79-06-1) | Glentham Life Sciences

10/7/2021 · Buy GK0096 – Acrylamide, 98% (79-06-1) online from Glentham Life Sciences, a manufacturer and supplier of fine chemicals. View catalogue prices, chemical data, technical

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LDS-sample buffer (4X), non-reducing – J61894 – Alfa Aesar

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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110-26-9 – N,N'-Methylenebisacrylamide, 99+% – Bis-acrylamide – BAC – 43701 – Alfa Aesar

Kinetics of polymerization of N,N-methylenebisacrylamide initiated by KMnO4-H2C2O4 redox system. Eur. Polym. J. 1982, 18, (6), 531-534. GHS Hazard and Precautionary Statements. Hazard Statements: H301-H312-H340-H350-H361-H372. Toxic if

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Clostridioides difficile (Prevot) Lawson et al. | ATCC

The certificate of analysis for that lot of Clostridioides difficile (Prevot) Lawson et al. ( 43593) is not currently available online. Complete this form to request this certificate of analysis. We have received your request for this

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