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Introduction to Polyacrylamide Gels | LSR | Bio-Rad

Polyacrylamide gels are characterized by two parameters: total monomer concentration (%T, in g/100 ml) and weight percentage of crosslinker (%C). By varying these two parameters, the pore size of the gel can be optimized to yield the best

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Migration of dyes Picture of Loading dye Cat. # Size, Composition Features Applications (1% agarose, tracking TAE or TBE buffers) dyes* 6X

polyacrylamide gels. Xylene cyanol FF: TAE: 4160 bp TBE: 3030 bp Orange G: TAE/TBE: <50 bp 6X TriTrack DNA Loading Dye R1161 5×1 6X Solution s M-4RIS s bromophenol blue s xylene cyanol FF s orange G s GLYCEROL s M-%$4! P( adjusted with NaOH)

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Polyacrylamide (gel)

Find Polyacrylamide (gel) and related products for scientific research at MilliporeSigma Research. Development. Production. We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology

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Purification and characterization of chalcone isomerase from soybeans – PubMed

Chalcone isomerase from soybean has been purified 11,000-fold over the crude extract. The purification procedure features pseudo-affinity chromatography on an Amicon Matrex Orange A column with selective elution by a product of the enzymatic

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runSAFE stain 1ml/vial | Cleaver Scientific

polyacrylamide gels. It is the most sensitive stain available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the

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Blue/Orange Loading Dye, 6X – Promega Corporation

3ml. Blue/Orange Loading Dye, 6X, is a convenient marker dye containing 0.4% orange G, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 15% Ficoll® 400, 10mM Tris-HCl (pH 7.5) and 50mM EDTA (pH 8.0). It is provided in a premixed, ready-to-use

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Polyacrylamide gel electrophoresis

Find polyacrylamide gel electrophoresis and related products for scientific research at MilliporeSigma Research. Development. Production. We are a leading supplier to the global Life Science industry with solutions and services for research,

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DirectLoad™ PCR 100 bp Low Ladder ready-to-use marker for DNA electrophoresis

Features and Benefits Ready-to-load Easy-to-use Popular band sizes Components Sigma′s DirectLoad PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol

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Polyacrylamide

Find polyacrylamide and related products for scientific research at MilliporeSigma Product Number Product Description SDS GF63566455 (hydrogel), granule, 2.5 mm nominal granule size, weight 500 g Pricing GF90890466 (hydrogel), granule, 2.5 mm

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Gel Documentation Systems | Thermo Fisher Scientific – HK

Invitrogen gel documentation systems accommodate a wide range of samples and support different modes of detection. Find the right features for your needs in our full line of gel doc systems. Safer visualization of nucleic acids-the blue or green

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DNA Gel Extraction Kit: QIAquick Gel Extraction Kit – QIAGEN

QIAquick Gel Extraction Kit (1000) For gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml) 28706X4. £1,353.00. Add to cart. QIAquick PCR & Gel Cleanup Kit (100) For purification PCR

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GeneRuler 100 bp DNA Ladder – Thermo Fisher Scientific

Thermo Scientific GeneRuler 100 bp DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 1,000 bp on agarose or polyacrylamide gels. The DNA ladder consists of 10 DNA fragments and

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Acrylamide

Acrylamide (or acrylic amide) is an organic compound with the chemical formula CH2=CHC(O)NH2. It is a white odorless solid, soluble in water and several organic solvents. From the chemistry perspective, acrylamide is a vinyl-substituted primary

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Electrophoresis – WUR

Electrophoresis usually is at about 5 Volts per cm for 0.5 – 2 hours or more at room temperature, depending on the desired separation. Size markers may be co-electrophoresed with DNA samples, when appropriate for fragment size determination.

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Affinity electrophoresis

Affinity-trap polyacrylamide gel electrophoresis Affinity-trap polyacrylamide gel electrophoresis (PAGE) has become one of the most popular methods of protein separation. This is not only due to its separation qualities, but also because it can

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DNA Gel Loading Dye | NEB

Gel Loading Dye, Purple (6X) is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis

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Nucleic Acid Electrophoresis Workflow—5 Main Steps | Thermo Fisher Scientific – US

Gel % (w/v) = (grams of agarose / milliliters of buffer) x 100% If a fluorescent nucleic acid stain is used, it may be included at a recommended concentration (e.g., 0.5 μg/mL of ethidium bromide) when casting the gel (learn more: Gel visualizat

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Hard and soft G

In the Latin-based orthographies of many European languages, the letter g is used in different contexts to represent two distinct phonemes that in English are called hard and soft g .The sound of a hard g (which often precedes the non-front

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DirectLoad™ 50 bp DNA Step Ladder ready-to-use marker for DNA electrophoresis

Features and Benefits Ready-to-load Easy-to-use Popular band sizes Components Sigma′s DirectLoad 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol

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DNA Gel Loading Dye | NEB

Gel Loading Dye, Purple (6X) is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis

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