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Introduction to Polyacrylamide Gels | LSR | Bio-Rad

Polyacrylamide gels are characterized by two parameters: total monomer concentration (%T, in g/100 ml) and weight percentage of crosslinker (%C). By varying these two parameters, the pore size of the gel can be optimized to yield the best

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Polyacrylamide Reagents and Precast Gels | Life Science Education | Bio-Rad

Gel opening lever ( 456-0000 ), sold separately, is 100% aluminum and recyclable. Ready Gel polyacrylamide precast gels are designed to fit the Mini-PROTEAN ® Tetra cell and are ready to run. Simply lock them into the cell, load your samples,

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Running agarose and polyacrylamide gels

17/6/2011 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be

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Electrophoresis and Blotting | Life Science Research | Bio-Rad

A guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more. Scroll Get Help Call us at 1-800-4-BIORAD (1-800-424-6723) Email a quick question Find local contacts Get product

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Criterion™ IEF Precast Gels | Life Science Research | Bio-Rad

IEF gels contain no denaturing agents, allowing one-dimensional separation under native conditions. Criterion precast gels include a broad selection of 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducibl

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TAE Buffer for agarose DNA electrophoresis

Tris-Acetate-EDTA (TAE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but also in agarose and polyacrylamide gel preparation. Also described in the literature is TAE's role in denaturing gradient gel electro

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Laemmli SDS sample buffer, reducing (4X) – J60015 – Alfa Aesar

Further, it is employed in the polyacrylamide gel analysis of proteins. In addition to this, it is utilized to reduce disulfide bonds in order to denature proteins. Notes Contains 250mM Tris-HCl(pH 6.8), 8% SDS, 40% glycerol, 8%

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Protocols – Western Transfer (Western Blot)

Protocols – Western Transfer (Western Blot) Western Transfer, also known as Western Blotting, is a rapid immunoblotting technique for identifying the presence of a particular protein in a complex mixture of proteins such as cell lysates or sera.

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TBE Buffer, 10X, Molecular Biology Grade | Tris-Borate-EDTA Buffer | Tris Buffer – Promega

TBE Buffer, 10X (pH 8.3), is used for polyacrylamide and agarose gel electrophoresis. This product is optimized for use in DNA applications. Form: Clear, colorless liquid. Composition: 890mM Tris-borate, 890mM boric acid, 20mM EDTA.

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Laemmli SDS sample buffer, reducing (6X) – J61337 – Alfa Aesar

Applications. For protein sample preparation to be used in the Laemmli SDS-PAGE system. Laemmli SDS sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Further, it is used for pol

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Blue native electrophoresis protocol | Abcam

Blue native polyacrylamide gel electrophoresis (BN-PAGE) is performed essentially as described by Schä gger and von Jagow (1991), Analytical Biochemistry, 199, 223-31. First, solubilized samples are stained with a charged (Coomassie) dye.

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30% Acrylamide/Bis Solution, 19:1 #1610154 | Life Science Research | Bio-Rad

Description. SDS. Use this 30% acrylamide/bis-acrylamide, 19:1 (5% crosslinker) solution as a faster and safer alternative to handling powdered acrylamide and bis-acrylamide. Ready-to-use high-purity (99.9%) solution. Reduce inhalation and

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Laemmli SDS sample buffer, non-reducing (6X) – J60660 – Alfa Aesar

Laemmli SDS sample buffer, non-reducing (6X) is an electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Composition of this buffer is similar to the reducing buffer minus mercaptoethanol. This product is

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SDS Solution, Molecular Biology Grade (10% w/v)

SDS Solution, Molecular Biology Grade (10% w/v) 100ml. $ 42.00. Your price: Log in. Change Configuration. SDS Solution (10% w/v) is sodium dodecyl sulfate in distilled, deionized water. SDS is a detergent that is known to denature proteins. It

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SYBR® Green I nucleic acid gel stain 10,000 × in DMSO

SYBR Green I is also a very sensitive stain for oligonucleotides, allowing for detection of as little as 1-2 ng of a synthetic 24-mer on a 5% polyacrylamide gel, which is 50-100 times greater sensitivity than obtained with ethidium bromide.

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TBE Buffer for Agarose Gel Electrophoresis

TBE Buffer (5X) is a solution used in Agarose Gel Electrophoresis (AGE) typically for the separation of nucleic acids (i.e. DNA and RNA). Tris-Borate-EDTA (TBE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but

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